Introduction of loxP sites by electroporation in the mouse genome; a simple approach for conditional allele generation in complex targeting loci

Table 3 Details of the projects successfully completed using the consecutive sequential electroporation procedure

Gene name	Procedure	Number of embryos electroporated	Number of embryos implanted	Number of surgeries	Number of gestations	Number of pups born	Percentage of live born animals (%)*	Number of pups properly targeted	Targeting efficiency (%)**
Icam1	Consecutive sequential electroporation	309	252	10	7	24	10	2	8
Lox	Consecutive sequential electroporation	159	140	5	4	22	16	1	5
Sar1b	Consecutive sequential electroporation	196	128	5	2	8	6	1	13

*Calculated by dividing the number of pups born by the number of implanted embryos
**Calculated by dividing the number of properly targeted pups by the total number of pups born

ISSN: 1472-6750