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Fig. 3 | BMC Biotechnology

Fig. 3

From: Introduction of loxP sites by electroporation in the mouse genome; a simple approach for conditional allele generation in complex targeting loci

Fig. 3

Non-consecutive sequential electroporation was successfully applied to generate Clcf1 floxed animals. A Schematic representation highlighting the primer positions and genotyping strategy used for characterization of Clcf1 F0 animals. PCR results from primers 2–6 combination are depicted in the upper-right panel. The sequencing results using primers 3 and 6 are highlighted below. PCR results from primers 1–7 combination are depicted in the lower panel. The sequencing results using primer 1 are highlighted below. B Schematic representation highlighting the primer positions and genotyping strategy used for characterization of Clcf1 floxed F0 animals. PCR results from primers 2–5 combination are depicted in the upper middle panel. PCR results and sequencing alignment from primers 1–5 combination are depicted in the lower left panel. PCR results and sequencing alignment from primers 2–6 combination are depicted in the lower right panel. Primers used for sequencing are highlighted in red. The same strategy was applied to complete a total of four different projects (Loxl1, Pard6a, Clcf1, Mapkap5)

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BMC Biotechnology

ISSN: 1472-6750

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