Method for quantification of porcine type I interferon activity using luminescence, by direct and indirect means

BMC Biotechnology

Table 2 Cytopathic effect inhibition assay with IFNβ and SGLuc-IFNβ samples

Sample	No. samples with vesicular stomatitis virus plaques in diluted growth media
Sample	1:100	1:200	1:400	1:800	1:1600	1:3200	1:6400	1:12,800
IFNβ	0/4	0/4	0/4	0/4	0/4	2/4	4/4	4/4
SGLuc-IFNβ	0/4	4/4	4/4	4/4	4/4	4/4	4/4	4/4

For determination of anti-viral activity for IFNβ and SGLuc-IFNβ samples, four wells for each dilution were evaluated for the presence of VSV plaques at 24 h post-infection. Wells were scored positive when plaques were observed

ISSN: 1472-6750