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Table 1 Activities of SGLuc-Δ1D2A-IFNα and IFNα-Δ1D2A-SGLuc Δ1M

From: Method for quantification of porcine type I interferon activity using luminescence, by direct and indirect means

 

Concentration measured by ELISA

(μg/mL; ± SD)

Luminescence

(RLUs/0.5 s)

CEI Assay

IFNAA50 (log10)/100 µl

Average

SD

24 h

36 h

IFNα-Δ1D2A-SGLuc Δ1M

 Batch 1

5.58 ± 1.39

2.99 × 1010

1.14 × 109

4.85

4.78

 Batch 2

6.93 ± 1.97

3.23 × 1010

1.14 × 109

5.00

4.93

 Batch 3

2.27 ± 0.84

1.31 × 1010

5.81 × 108

4.55

4.55

SGLuc-Δ1D2A-IFNα

 Batch 1

9.89 ± 1.15

2.61 × 1010

5.78 × 108

5.15

5.08

 Batch 2

9.56 ± 1.20

2.13 × 1010

6.05 × 108

5.30

5.15

 Batch 3

11.68 ± 3.98

2.40 × 1010

7.30 × 108

5.30

5.15

  1. Concentration, luminescence, and anti-VSV activity for three batches of SGLuc-Δ1D2A-IFNα and IFNα-Δ1D2A-SGLuc Δ1M. Concentration was determined by porcine IFNα ELISA, luminescence by reactivity with coelenterazine (N = 3 replicates per sample), and anti-VSV activity by CEI (N = 4 replicates per sample). RLUs/0.5 s, relative luciferase units per half second; CEI, Cytopathic effect inhibition assay; IFNAA50/100 μL, Interferon Antiviral Activity per 100 μL
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BMC Biotechnology

ISSN: 1472-6750

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