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Table 1 Activities of SGLuc-Δ1D2A-IFNα and IFNα-Δ1D2A-SGLuc Δ1M

From: Method for quantification of porcine type I interferon activity using luminescence, by direct and indirect means

  Concentration measured by ELISA
(μg/mL; ± SD)
Luminescence
(RLUs/0.5 s)
CEI Assay
IFNAA50 (log10)/100 µl
Average SD 24 h 36 h
IFNα-Δ1D2A-SGLuc Δ1M
 Batch 1 5.58 ± 1.39 2.99 × 1010 1.14 × 109 4.85 4.78
 Batch 2 6.93 ± 1.97 3.23 × 1010 1.14 × 109 5.00 4.93
 Batch 3 2.27 ± 0.84 1.31 × 1010 5.81 × 108 4.55 4.55
SGLuc-Δ1D2A-IFNα
 Batch 1 9.89 ± 1.15 2.61 × 1010 5.78 × 108 5.15 5.08
 Batch 2 9.56 ± 1.20 2.13 × 1010 6.05 × 108 5.30 5.15
 Batch 3 11.68 ± 3.98 2.40 × 1010 7.30 × 108 5.30 5.15
  1. Concentration, luminescence, and anti-VSV activity for three batches of SGLuc-Δ1D2A-IFNα and IFNα-Δ1D2A-SGLuc Δ1M. Concentration was determined by porcine IFNα ELISA, luminescence by reactivity with coelenterazine (N = 3 replicates per sample), and anti-VSV activity by CEI (N = 4 replicates per sample). RLUs/0.5 s, relative luciferase units per half second; CEI, Cytopathic effect inhibition assay; IFNAA50/100 μL, Interferon Antiviral Activity per 100 μL