Fig. 2

A Diagrams of bi-cistronic SGLuc-Δ1D2A-IFNα and IFNα-Δ1D2A-SGLucΔ1M constructs evaluated for correlation of luciferase and antiviral activities. Pink box represents the IFNα secretion peptide sequence. B Western blots using anti-porcine IFNα and anti-GLuc antibodies demonstrated protein banding patterns with expected sizes and confirmed secretion into cell culture media. Blots were cropped to focus on results and representative of N = 2 replicates; uncropped full-length blots are included in Additional file 1: Fig. S1. C Luminescence of serially diluted media, N = 5, samples from cultures producing SGLuc-Δ1D2A-IFNα and IFNα-Δ1D2A-SGLuc Δ1M. Sample dilutions range from 1/50 to 1/6400. Dotted lines represent the line of best fit