Table 3 PCR settings for KAPA HiFi HotStart ReadyMix PCR Kit
Step | Step I | Step III | ||
|---|---|---|---|---|
Cycles | Temperature/duration | Cycles | Temperature/duration | |
Initial denaturation | 1 | 95 °C/3 min | 1 | 95 °C/3 min |
Denaturation | 30 | 98 °C/20 s | 30 | 98 °C/20 s |
Annealing | 60/56 °C/20 s | 60/56 °C/20 s | ||
Extension | 72 °C/1 min | 72 °C/2 min | ||
Final extension | 1 | 72 °C/5 min | 1 | 72 °C/5 min |