Fig. 4.
From: Development of a CHO cell line for stable production of recombinant antibodies against human MMP9
A ELISA signals of anti-hMMP9 antibody from various colonies following a second round of selection; B SDS-PAGE analysis of expressed anti-hMMP antibodies from #6–5 and #6–9 clones with (+) or without (−) protein reducing; C SDS-PAGE analysis of #6–5 clone after PA affinity chromatography with (+) or without (−) protein reducing; and D ELISA signals of #6–5 clone and commercial anti-hMMP9 antibody with different concentrations of hMMP9. Error bars represent ±1 standard deviation (SD) (n = 3). We displayed cropped gels in Fig. 4B, C. The original gels were presented in Additional file 1: Fig. S6.