Fig. 2
OX5382 construct schematics and splicing. A Linear construct map for OX5382. The position of the conditional female-specific self-limiting gene and that of the fluorescent marker is indicated. B Predicted splicing pattern of OX5382. The mRNA is predicted to generate three major sex-specific splice forms: F1, F2 and M. The F1 and F2 female-specific splice forms are in-frame with tTAV, while the male-specific M isoform is not. Translation start sites are indicated by an arrow; in-frame stop codons are indicated by red octagons. The ubiquitin cleavage site in the translated F1 and F2 DSX proteins is indicated by scissors. C Representative mRNA splicing analysis on OX5382 G1 adults reared on tetracycline. Both endogenous Sfdsx (top gel) and construct-derived OX5382 Sfdsx show the predicted sex-specific splicing pattern. Non-injected wild-type individuals were included in the assay as a negative control for the construct-specific splicing PCR. NoRT: non-reverse transcribed control; NTC: no template control