Fig. 1
From: Enzymatic degradation is an effective means to reduce aflatoxin contamination in maize
Schematic of the construct used to transform maize. A synthetic construct was manufactured consisting of 1.4 kb section of the embryo-specific Zea mays globulin 1 promoter driving expression of an ER-targeted plant codon optimized 2.166 kb open reading frame encoding for Armillariella tabescenes’ aflatoxin-degrading enzyme. ER-targeting elements were added and consisted of a 5′ addition of a 22 amino-acid encoding signal sequence from the Arabidopsis chitinase gene and the 3′ addition of the ER retention KHDEL motif. Numbers are relative to indicate size of each construct element