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Table 1 The affinity of AK2 with CDK4 according to the non‐competitive ELISA assay

From: Expression, purification and characterisation of a human anti-CDK4 single-chain variable fragment antibody

Antibody

Affinity [× 107 M−1]

   
 

Kn=2

Kn=4

Kn=8

Mean ± SD

AK2

2.77, 2.28, 2.37

2.58, 2.31

2.55

2.48 ± 0.17

  1. Kn=2 is the collection of affinity calculated from every 2 curves differed in amount of coated CDK4 by twofold dilution. Analogized from this, Kn=4 and Kn=8 were calculated and listed above
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BMC Biotechnology

ISSN: 1472-6750

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