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Fig. 5 | BMC Biotechnology

Fig. 5

From: Expression, purification and characterisation of a human anti-CDK4 single-chain variable fragment antibody

Fig. 5

Binding activity of AK2 to CDK4 in cells as measured via an immunofluorescence assay. Cells were incubated sequentially with purified AK2, anti-V5 tag antibody then FITC-conjugated IgG as part of the immunofluorescence assay, with Hoechst33342 used to stain nucleus. Cells not incubated with purified AK2 served as the negative control (NC). A-F: HeLa cells; G-L: MCF-7 cells

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BMC Biotechnology

ISSN: 1472-6750

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