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Table 1 Cultivable biomass of L. plantarum CNCM I-4459 (CFU/mL) for each storage time depending on the lyoprotectant

From: Design of a new lyoprotectant increasing freeze-dried Lactobacillus strain survival to long-term storage

Storage time

Lyoprotectants

Sucrose

Sucrose + Micellar Casein

Sucrose + Whey

Sucrose + Inulin

0 month

1.9 × 1010

a

1.4 × 1010

a

1.8 × 1010

a

1.8 × 1010

a

 

 ± 2.5 × 109

 

 ± 1.9 × 109

 

 ± 1.2 × 109

 

 ± 1.8 × 109

 

0.5 month

1.6 × 1010

a

9.4 × 109

ab

4.8 × 109

b

1.1 × 1010

ab

 

 ± 2.0 × 109

 

 ± 2.1 × 109

 

 ± 9.7 × 108

 

 ± 6.6 × 109

 

1 month

1.1 × 1010

a

1.0 × 1010

a

7.9 × 108

b

1.6 × 109

b

 

 ± 4.6 × 109

 

 ± 2.0 × 109

 

 ± 7.7 × 108

 

 ± 6.4 × 108

 

3 months

1.5 × 109

ab

3.4 × 109

a

2.6 × 109

a

1.0 × 108

b

 

 ± 1.0 × 108

 

 ± 3.0 × 109

 

 ± 3.6 × 109

 

 ± 1.4 × 108

 

9 months

1.9 × 107

b

5.8 × 109

a

4.7 × 106

b

1.9 × 106

b

 

 ± 3.2 × 106

 

 ± 9.9 × 108

 

 ± 1.0 × 106

 

 ± 1.0 × 106

 
  1. Values represent mean ± standard deviation obtained from independent triplicates. Different letters indicate significant differences between lyoprotectants (Tukey’s Honest Significant Difference test, p < 0.05)