Fig. 3
From: Preparation of an anti-NEK2 monoclonal antibody and its application in liver cancer
Specificity detection of anti-NEK2 mAb. a Analysis of the antigenic specificity of anti-NEK2 mAb by Western blot. For improved clarity and conciseness, cropped areas of the blot are shown. The full length (uncut) blot image is shown in Additional file 2: Fig. S10. b Analysis of the antigenic specificity of the anti-NEK2 mAb by ELISA. c Detection of NEK2 subcellular localization by immunofluorescence assay at HCC cell lines HepG2, Huh7 and Hep3B, and the normal liver cell line HL7702. Cell nuclei were stained with DAPI (blue). d Detection of NEK2 expression in HCC cells by immunocytochemical staining at HCC cell lines HepG2, Hep3B and the normal liver cell line HL7702. e Quantitative analysis of immunocytochemical staining. f NEK2 mRNA relative expression at HCC cell lines HepG2, Hep3B and the normal liver cell line HL7702. g The expression levels of NEK2 between normal liver tissues and primary liver tumor tissues by TCGA database were analyzed