Fig. 2
From: Preparation of an anti-NEK2 monoclonal antibody and its application in liver cancer
Preparation of anti-NEK2 mAb. a Serum antibody titer of mouse after immunization with NEK2 protein was tested by iELISA. b SDS-PAGE analysis of 3A3 mAb purified. For improved clarity and conciseness, cropped areas of the gel are shown. The full length (uncut) gel image is shown in Additional file 2: Fig. S9. c The titer of purified anti-NEK2 3A3 mAb was tested by iELISA. d The isotype of purified anti-NEK2 3A3 mAb was tested by iELISA. e The affinity of purified anti-NEK2 3A3 mAb was tested by iELISA