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Table 2 Bioactivity of rec-eCG mutants in cells expressing ratFSH receptor

From: Roles of N-linked and O-linked glycosylation sites in the activity of equine chorionic gonadotropin in cells expressing rat luteinizing hormone/chorionic gonadotropin receptor and follicle-stimulating hormone receptor

rec-eCG cAMP responses
Basala (nM/104 cells) EC50b (ng/mL) Rmaxc (nM/104 cells)
rec-eCGβ/α-wt 0.4 ± 0.1 27.1 (1.0-fold) (20.2 to 40.8) d 16.5 ± 0.5 (1.0-fold)
rec-eCGβ/αΔ56 0.4 ± 0.1 111.5 (4.1-fold) (94.8 to 135.2) 5.5 ± 0.2 (0.33-fold)
rec-eCGβ-D/α 0.3 ± 0.1 63.1 (2.3-fold) (51.3 to 81.9) 14.2 ± 0.4 (0.86-fold)
rec-eCGβ-D/αΔ56 0.4 ± 0.1 94.9 (3.5-fold) (69.8 to 147.8) 5.4 ± 0.3 (0.32-fold)
  1. Values are the means ± SEM of triplicate experiments. EC50 values were determined from the concentration–response curves from in vitro bioassays. The cAMP responses of the basal and Rmax in rec-eCGβ/α-wild type were shown as onefold
  2. aBasal cAMP level average without agonist treatment
  3. bGeometric mean (95% confidence limit)
  4. cRmax average cAMP level/104 cells
  5. d95% Confidence intervals