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Table 2 Bioactivity of rec-eCG mutants in cells expressing ratFSH receptor

From: Roles of N-linked and O-linked glycosylation sites in the activity of equine chorionic gonadotropin in cells expressing rat luteinizing hormone/chorionic gonadotropin receptor and follicle-stimulating hormone receptor

rec-eCG

cAMP responses

Basala (nM/104 cells)

EC50b (ng/mL)

Rmaxc (nM/104 cells)

rec-eCGβ/α-wt

0.4 ± 0.1

27.1 (1.0-fold) (20.2 to 40.8) d

16.5 ± 0.5 (1.0-fold)

rec-eCGβ/αΔ56

0.4 ± 0.1

111.5 (4.1-fold) (94.8 to 135.2)

5.5 ± 0.2 (0.33-fold)

rec-eCGβ-D/α

0.3 ± 0.1

63.1 (2.3-fold) (51.3 to 81.9)

14.2 ± 0.4 (0.86-fold)

rec-eCGβ-D/αΔ56

0.4 ± 0.1

94.9 (3.5-fold) (69.8 to 147.8)

5.4 ± 0.3 (0.32-fold)

  1. Values are the means ± SEM of triplicate experiments. EC50 values were determined from the concentration–response curves from in vitro bioassays. The cAMP responses of the basal and Rmax in rec-eCGβ/α-wild type were shown as onefold
  2. aBasal cAMP level average without agonist treatment
  3. bGeometric mean (95% confidence limit)
  4. cRmax average cAMP level/104 cells
  5. d95% Confidence intervals
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BMC Biotechnology

ISSN: 1472-6750

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