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Table 1 Bioactivity of rec-eCG mutants in cells expressing ratLH/CG receptor

From: Roles of N-linked and O-linked glycosylation sites in the activity of equine chorionic gonadotropin in cells expressing rat luteinizing hormone/chorionic gonadotropin receptor and follicle-stimulating hormone receptor

rec-eCG

cAMP responses

Basala (nM/104 cells)

EC50b (ng/mL)

Rmaxc (nM/104 cells)

rec-eCGβ/α-wt

0.4 ± 0.1

4.6 (1.0-fold) (3.6 to 6.3)d

23.1 ± 0.5 (1.0-fold)

rec-eCGβ/αΔ56

0.3 ± 0.1

17.63 (3.8-fold) (13.9 to 24.1)

11.6 ± 0.3 (0.50-fold)

rec-eCGβ-D/α

0.3 ± 0.1

24.5 (5.3-fold) (20.9 to 29.6)

20.3 ± 0.4 (0.88-fold)

rec-eCGβ-D/αΔ56

0.2 ± 0.1

334.2 (72.7-fold) (282.4 to 409.1)

10.3 ± 0.4 (0.45-fold)

  1. Values are the means ± SEM of triplicate experiments. EC50 values were determined from the concentration–response curves from in vitro bioassays. The cAMP responses of the basal and Rmax in rec-eCGβ/α-wild type were shown as onefold
  2. aBasal cAMP level average without agonist treatment
  3. bGeometric mean (95% confidence limit)
  4. cRmax average cAMP level/104 cells
  5. d95% Confidence intervals
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BMC Biotechnology

ISSN: 1472-6750

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