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Table 4 Amplification efficiency of tested miRNAs at optimized annealing temperature (n = 3)

From: Screening of miRNAs in plasma as a diagnostic biomarker for cardiac disease based on optimization of extraction and qRT-PCR condition assay through amplification efficiency

miRNAs Annealing temperature (°C) Median Efficiency (%) Range 95% CI
Cel-miRNA-39-1 60 91.2 99.0–89.8 81.1–105.4
miRNA-16 65 97.6 107.6–93.0 80.9–117.9
miRNA-21 65 98.1 106.3–96.4 87.1–113.4
miRNA-132 60 95. 2 95.8–85.2 77.3–106.9
miRNA-155 65 108.7 112.7–86.5 67.6–137.7
miRNA-499 65 94.9 94.6–95.4 93.9–96.0