Table 4 Amplification efficiency of tested miRNAs at optimized annealing temperature (n = 3)
miRNAs | Annealing temperature (°C) | Median Efficiency (%) | Range | 95% CI |
|---|---|---|---|---|
Cel-miRNA-39-1 | 60 | 91.2 | 99.0–89.8 | 81.1–105.4 |
miRNA-16 | 65 | 97.6 | 107.6–93.0 | 80.9–117.9 |
miRNA-21 | 65 | 98.1 | 106.3–96.4 | 87.1–113.4 |
miRNA-132 | 60 | 95. 2 | 95.8–85.2 | 77.3–106.9 |
miRNA-155 | 65 | 108.7 | 112.7–86.5 | 67.6–137.7 |
miRNA-499 | 65 | 94.9 | 94.6–95.4 | 93.9–96.0 |