Skip to main content

Table 1 Comparison of slop, amplification efficiency, and R2 estimated through the linear regression of triple standard curves of miRNA-16 according to the annealing temperature

From: Screening of miRNAs in plasma as a diagnostic biomarker for cardiac disease based on optimization of extraction and qRT-PCR condition assay through amplification efficiency

Parameters Annealing temp. (℃)
60 62 65 67
Slope
 Median − 3.363 − 3.751 − 3.380 − 3.724
 Range − 4.3555 to − 3.6579 − 3.8144 to − 3.5217 − 3.5037 to − 3.1532 − 3.7350 to − 3.5115
 95% CI − 4.849 to − 3.068 − 4.078 to − 3.318 − 3.787 to − 2.904 − 3.970 to − 3.344
Efficiency (%)
 Median 81.48 84.76 97.62 85.57
 Range 69.7 to 81.5 82.9 to 92.3 92.9 to 107.6 85.2 to 92.7
 95% CI 56.89 to 102.3 74.27 to 99.01 80.82 to 117.9 77.42 to 98.23
R2
 Median 0.9985 0.9995 0.9987 0.9914
 Range 0.9902 to 0.9996 0.9966 to 0.9995 0.9981 to 0.9998 0.9914 to 0.9995
 95% CI 0.9833 to 1.009 0.9943 to 1.003 0.9967 to 1.001 0.9966 to 1.009