Table 1 Comparison of slop, amplification efficiency, and R² estimated through the linear regression of triple standard curves of miRNA-16 according to the annealing temperature

− 3.363

− 3.751

− 3.380

− 3.724

− 4.3555 to − 3.6579

− 3.8144 to − 3.5217

− 3.5037 to − 3.1532

− 3.7350 to − 3.5115

− 4.849 to − 3.068

− 4.078 to − 3.318

− 3.787 to − 2.904

− 3.970 to − 3.344

81.48

84.76

97.62

85.57

69.7 to 81.5

82.9 to 92.3

92.9 to 107.6

85.2 to 92.7

56.89 to 102.3

74.27 to 99.01

80.82 to 117.9

77.42 to 98.23

0.9985

0.9995

0.9987

0.9914

0.9902 to 0.9996

0.9966 to 0.9995

0.9981 to 0.9998

0.9914 to 0.9995

0.9833 to 1.009

0.9943 to 1.003

0.9967 to 1.001

0.9966 to 1.009