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Fig. 5 | BMC Biotechnology

Fig. 5

From: Screening of miRNAs in plasma as a diagnostic biomarker for cardiac disease based on optimization of extraction and qRT-PCR condition assay through amplification efficiency

Fig. 5

Efficiency of RNA extraction for endogenous miRNA-16 detection from plasma by qRT-PCR with number of chloroform extractions and with or without ethanol washing. Results represent raw Cq values obtained for miRNA-16. RNA samples from plasma were obtained by extraction with Trizol LS in transferring 400 uL of upper phase after phase separation. E1: Once chloroform extraction without EtOH washing, E2: Once chloroform extraction with EtOH washing, E3: Triple chloroform extraction without EtOH washing, E4: Triple chloroform extraction with EtOH washing. In the figure, dots represent individual Cq values, the horizontal bar is the group mean. Statistical differences were determined using Kruskal–Wallis test followed by Dunn’s post hoc test for multiple comparisons (*P = 0.0289)

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