Fig. 2

Binding of anti-hCCR6 mAb to RBL transfected cells with hCCR6. a–d Comparison of the reactivity against mock cells and HA-hCCR6-expressing RBL cells between control isotype a, KM4703 b, 1C6 c and HA d antibodies at 20 μg/mL. e–h Representative flow cytometry analysis of RBL-hCCR6 and mock cells stained with different doses of mAb 1C6 (1–10 μg/mL). Blue histograms of hCCR6⁺ cells are superimposed over grey histograms from mock transfectans. One representative analysis from three independent experiments is shown. The histogram overlays were performed using BD CellQuest Pro Software