Table 1 DNA extraction protocols tested on six individual adult, female T. circumcincta specimens per method in this study
Method or kit name | Protocol code | Reference or supplier (catalogue #) | Extraction method | Time required (hours) |
|---|---|---|---|---|
AccuPrep® Genomic DNA Extraction - Mammalian Tissue | AccM | Bioneer (K-3032) | Silica binding | 1.5 |
AccuPrep® Genomic DNA Extraction Kit - Whole Blood, Buffy Coat and Cultured Cells | AccW | Bioneer (K-3032) | Silica binding | 0.5 |
Chelex®100 | CheX | PS Walsh et al. [20] | Chelating | 0.5 |
Cetyl Trimethyl Ammonium Bromide | CTAB | T Sarkinen et al. [23] | Precipitation | 6 |
E.Z.N.A.® Forensic DNA | EznF | Omega Bio-tek (D3591–00) | Silica binding | 1.5–2 |
Isolate II Genomic DNA Kit | IsoG | Bioline (BIO-52066) | Silica binding | 1.5–3 |
Schistosoma sp. DNA Extraction Method | Schi | PJ Brindley et al. [24] | Precipitation | 1.5 |
Sodium Dodecyl Sulphate | SDS | K Edwards et al. [25] | Precipitation | 1.25–1.75 |
Wizard® Genomic DNA Purification Kit - Mouse Tail | WizM | Promega (A1120) | Precipitation | 4–4.5a |
Wizard® Genomic DNA Purification - Plant Tissue | WizP | Promega (A1120) | Precipitation | 1a |
Larval exsheathment | -LE | HJ Dawkins et al. [26] | – | 1 |