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Table 1 DNA extraction protocols tested on six individual adult, female T. circumcincta specimens per method in this study

From: Comparative evaluation of different molecular methods for DNA extraction from individual Teladorsagia circumcincta nematodes

Method or kit name Protocol code Reference or supplier (catalogue #) Extraction method Time required (hours)
AccuPrep® Genomic DNA Extraction - Mammalian Tissue AccM Bioneer (K-3032) Silica binding 1.5
AccuPrep® Genomic DNA Extraction Kit - Whole Blood, Buffy Coat and Cultured Cells AccW Bioneer (K-3032) Silica binding 0.5
Chelex®100 CheX PS Walsh et al. [20] Chelating 0.5
Cetyl Trimethyl Ammonium Bromide CTAB T Sarkinen et al. [23] Precipitation 6
E.Z.N.A.® Forensic DNA EznF Omega Bio-tek (D3591–00) Silica binding 1.5–2
Isolate II Genomic DNA Kit IsoG Bioline (BIO-52066) Silica binding 1.5–3
Schistosoma sp. DNA Extraction Method Schi PJ Brindley et al. [24] Precipitation 1.5
Sodium Dodecyl Sulphate SDS K Edwards et al. [25] Precipitation 1.25–1.75
Wizard® Genomic DNA Purification Kit - Mouse Tail WizM Promega (A1120) Precipitation 4–4.5a
Wizard® Genomic DNA Purification - Plant Tissue WizP Promega (A1120) Precipitation 1a
Larval exsheathment -LE HJ Dawkins et al. [26] 1
  1. a: Additional overnight incubation required