Fig. 2From: Production of IgY polyclonal antibody against diphtheria toxin and evaluation of its neutralization effect by Vero cell assayAnalysis of the PEG purified anti-DT IgY. a SDS-PAGE analysis. The IgY samples were resolved on 12% gel and stained by Coomassie Brilliant Blue R-250. b Western blot analysis. The IgY samples were run on a 12% SDS-PAGE gel and transferred onto a nitrocellulose membrane. The samples were probed by peroxidase-conjugated rabbit anti-chicken IgY. 1: Non-reducing condition. 2. Molecular weight marker. 3: Reducing conditionBack to article page