From: Rational design and structure-based engineering of alkaline pectate lyase from Paenibacillus sp. 0602 to improve thermostability
Enzyme
Specific activity (U/mg)
t1/2 (60 °C) (min)
Optimum temperature (°C)
Wild type
1602 ± 124
33.47 ± 2.02
67.5
G241A
1449 ± 94
61.86 ± 3.94
G241V
1704 ± 119
47.09 ± 3.49
K93I/G241A
2707 ± 64
49.33 ± 0.14
60