Fig. 3
Calibration curves for the transcripts of 35 genes as amplified by GeXP RT-PCR in the same tube. A sample of a total RNA pool from light-induced and control plasmodial cells (see Fig. 2) was serially diluted and the 35 cDNA fragments quantified after capillary electrophoretic separation. Each RNA concentration was analysed twice and each of the obtained data points, normalized peak area (NPA) versus concentration of total RNA, was displayed in a double logarithmic plot (logarithm to the base 10). All data points were fitted to a straight line of the same slope as described in Materials and Methods. The R2 value displayed in each panel gives the correlation coefficient of the first linear regression fit (see Methods for details). The red line in each panel represents the straight line of equal slope finally fitted to the data for each of the 35 transcripts (second linear regression fit; see Methods for details). For names of genes see Table S1. The figure was prepared with R, version 3.5.2