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Fig. 1 | BMC Biotechnology

Fig. 1

From: In vitro assessment of two novel Cellulases from Trabulsiella odontotermitis for agricultural waste utilization

Fig. 1

SDS-PAGE analysis of protein overexpression (a) recombinant egl-FZYE. Lane M: Molecular weight markers 250 kDa; Lane 1: cell extract before isopropyl-β-D-1-thiogalactopyranoside (IPTG) induction; Lane 2: cell extract after IPTG induction; Lane 4: cell extract pellet; Lane 5: cell extract supernatant; Lane 6: Resin before fusion protein cleavage reaction; Lanes 7–12: Elution of target protein (arrow). The complete picture is available in Supplementary Fig. 1. b recombinant cel-FZYE. Lane M: Molecular weight markers 250 kDa; Lane 1: cell extract before IPTG induction; Lane 2: cell extract after IPTG induction; Lane 3: cell extract pellet; Lane 4: cell extract supernatant; Lane 5: Resin before fusion protein cleavage reaction; Lanes 6–8: Elution of target protein (arrow). The complete picture is available in Supplementary Fig. 2

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BMC Biotechnology

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