Fig. 2From: Construction and characterization of a high-quality cDNA library of Cymbidium faberi suitable for yeast one- and two-hybrid assaysThe synthesis, purification and normalization of cDNA. a. the purification of mRNA from total RNA; b. the double-strand cDNA synthesized by LD-PCR; c. the purification of cDNA using a CHROMA SPIN TE-400 column; d. the normalization of the ds cDNA. M: DL 10000 DNA Marker; 1: mRNA or cDNA from C. faberi; 2: positive controlBack to article page