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Table 2 Buffers used in protein purification

From: Intein-mediated recombinant expression of monomeric B22Asp desB30 insulin

BuffersDetails
Buffer A50 mM Tris-HCl, 2 mM EDTA, 100 mM NaCl
Buffer B50 mM Tris-HCl, 1 mM EDTA, 100 mM NaCl,1% Tween
Buffer C50 mM Tris-HCl, 1 mM EDTA, 100 mM NaCl, 1%
Tween, 4 M Urea
Buffer D50 mM Tris-HCl, 1 mM EDTA, 100 mM NaCl, 3% Tween
Buffer E50 mM Tris-HCl, 1 mM EDTA, 100 mM NaCl,0.5%
Tween
Buffer F50 mM Tris-HCl, 1 mM EDTA, 10 mM β-
Mercaptoethanol, 2 mM Sodium deoxycholate pH 8.0, 8
M Urea
TGE buffer50 mM Tris-HCl, 0.5 mM EDTA,50 mM NaCl, 5%
Glycerol, 1% Glycine, 0.1% Glutathione pH 8.0
Digestion buffer20 mM Tris–HCl, 200 mM NaCl and 1 mM EDTA,
pH 8.0
Buffer G0.1% TFA
Buffer H90% HPLC grade acetonitrile in H2O, 0.08% TFA