Table 2 Buffers used in protein purification
From: Intein-mediated recombinant expression of monomeric B22Asp desB30 insulin
Buffers | Details |
|---|---|
Buffer A | 50 mM Tris-HCl, 2 mM EDTA, 100 mM NaCl |
Buffer B | 50 mM Tris-HCl, 1 mM EDTA, 100 mM NaCl,1% Tween |
Buffer C | 50 mM Tris-HCl, 1 mM EDTA, 100 mM NaCl, 1% Tween, 4 M Urea |
Buffer D | 50 mM Tris-HCl, 1 mM EDTA, 100 mM NaCl, 3% Tween |
Buffer E | 50 mM Tris-HCl, 1 mM EDTA, 100 mM NaCl,0.5% Tween |
Buffer F | 50 mM Tris-HCl, 1 mM EDTA, 10 mM β- Mercaptoethanol, 2 mM Sodium deoxycholate pH 8.0, 8 M Urea |
TGE buffer | 50 mM Tris-HCl, 0.5 mM EDTA,50 mM NaCl, 5% Glycerol, 1% Glycine, 0.1% Glutathione pH 8.0 |
Digestion buffer | 20 mM Tris–HCl, 200 mM NaCl and 1 mM EDTA, pH 8.0 |
Buffer G | 0.1% TFA |
Buffer H | 90% HPLC grade acetonitrile in H2O, 0.08% TFA |