Fig. 6
From: Intein-mediated recombinant expression of monomeric B22Asp desB30 insulin
Purification and mass spectroscopy confirm B22D desb30 insulin. a: HPLC of B22D desB30 insulin. The UV absorption was detected at 280 nm. Conditions: equipment, HPLC Waters 600 (Waters, USA). Column: Kromasil C8 column (4.6 × 250 mm). Mobile phase: buffer G (0.1% TFA), buffer H (90% HPLC grade acetonitrile in H2O, 0.08% TFA). Gradient: 35–50% buffer B in 30 min. Flow rate: 1 ml/min. Injected volume: 200 μl. b: mass spectroscopy analysis of B22D desB30 insulin. The determined MW is 5664.0 Da