Table 1 Effect of various chemical agents on keratinase stability

None

–

100 ± 4.24^d

EDTA

5 mM

0 ± 0^a

1,10-phenanthroline

5 mM

0 ± 0^a

PMSF

5 mM

89 ± 6.22^c

Sodium thioglycolate

5 mM

64 ± 2.70^b

Dithiothreitol

5 mM

69 ± 0.09^b

Sodium sulfite

5 mM

78 ± 3.61^c

2-Mercaptoethanol

5 mM

111 ± 0.54^e

Hydrogen peroxide

1% (v/v)

103 ± 3.52^d

Dimethyl sulfoxide

1% (v/v)

114 ± 5.86^e

Acetonitrile

1% (v/v)

61 ± 3.34^b

Propan-2-ol

1% (v/v)

79 ± 3.97^c

Sodium dodecyl sulfate

5 mM

77 ± 4.51^c

Triton X-100

1% (v/v)

99 ± 2.16^d

Tween-80

1% (v/v)

182 ± 1.98^f