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Table 4 Effects of metal ions on fibrinolytic enzyme activity

From: An effective combination of codon optimization, gene dosage, and process optimization for high-level production of fibrinolytic enzyme in Komagataella phaffii (Pichia pastoris)

Metal ions Concentration relative activity (%)a
5 mM 20 mM 50 mM
Ca2+ 117.5 ± 1.8 121.2 ± 2 97.1 ± 1.2
Mg2+ 100.5 ± 1 108.9 ± 1.4 98.0 ± 2.1
Zn2+ 8.1 ± 1.4 4.5 ± 1.2 1.8 ± 1.0
Cu2+ 5.4 ± 1.9 0.0 ± 1.0 0
Mn2+ 88.3 ± 1.6 7.3 ± 1.4 0
Pb2+ 0 0 0
Fe3+ 0 0 0
CKb 100 100 100
  1. aThe relative activity with different metal ions added was determined and compared with the activity measured in phosphate buffer (pH 7.4, 0.02 M) without the addition of any ions
  2. bThe relative activity measured in phosphate buffer (pH 7.4, 0.02 M) without the addition of any ions
  3. All values are mean ± SD from three independent experiments