Fig. 4

Cultivation optimization of recombinant strain GS115/pPIC9K-fib in shaking flasks. The figures show the effect of different media and days (a), induction temperatures (b), methanol concentrations (c), coculture with methanol and sorbitol (d) on enzyme activity (column), growth (line and triangle), and protein concentration (line and circular), respectively. All values are mean ± SD from three independent experiments