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Table 5 Details of assay reactions for determination of enzyme activity and inhibition assays in the presence of various dilutions of P. sidoides extract

From: Novel kinase platform for the validation of the anti-tubercular activities of Pelargonium sidoides (Geraniaceae)

Enzyme Enzyme Activity Assay reaction mixtures P. sidoides inhibition Assay reaction mixtures Incubation time
NDK 100 mM K-PO4 buffer (pH 6.8), 250 mM KCl
5 nM enzyme, 0.2 M Thymidine diphosphate
100 mM K-PO4 buffer (pH 6.8), 250 mM KCl
5 nM enzyme, 0.2 M Thymidine diphosphate
40 mins
HSK 50 mM HEPES buffer (pH 7.0), 450 mM KCl
704 nM enzyme, 10 mM Homoserine
50 mM HEPES buffer (pH 7.0), 450 mM KCl
704 nM enzyme, 10 mM Homoserine
4 h
AK 100 mM Tris buffer (pH 7.0), 250 mM KCl
223 nM enzyme, 10 mM Na-acetate
100 mM Tris buffer (pH 7.0), 250 mM KCl
223 nM enzyme, 10 mM Na-acetate
24 h
GK 100 mM Tris buffer (pH 7.0), 250 mM KCl
208.6 nM enzyme, 100 mM Glycerol
100 mM Tris buffer (pH 7.0), 250 mM KCl
208.6 nM enzyme, 100 mM Glycerol
24 h
ThiL 100 mM Tris buffer (pH 8.0), 250 mM KCl
2074 nM enzyme, 1 mM Thiamine monophosphate
100 mM Tris buffer (pH 8.0), 250 mM KCl
2074 nM enzyme, 1 mM Thiamine monophosphate
5 h
RBKS 100 mM Tris buffer (pH 7.2), 100 mM KCl
250 nM enzyme, 10 mM d-ribose
100 mM Tris buffer (pH 7.2), 100 mM KCl
250 nM enzyme, 10 mM d-ribose
4 h
AsK 100 mM Tris-HCl buffer (pH 7.5), 178.2 nM enzyme
10 mM l-Aspartic acid
100 mM Tris-HCl buffer (pH 7.5), 178.2 nM enzyme
10 mM l-Aspartic acid
6 h
SK 100 mM K-PO4 buffer (pH 6.8), 500 mM KCl
10 nM enzyme, 8 mM shikimic acid
100 mM K-PO4 buffer (pH 6.8), 500 mM KCl
10 nM enzyme, 8 mM shikimic acid
20 mins