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Table 4 Natural levels and interval of variation in the independent variables in the design of cellulose production

From: Optimized culture conditions for bacterial cellulose production by Acetobacter senegalensis MA1

Factors

Codes

Levels

Interval of variation

-1

0

+ 1

Carbon

Glycerol (mL L− 1)

45.0

50.0

55.0

5.0

Nitrogen

Yeast extract (g L− 1)

5.0

7.5

10.0

2.5

pH

 

4.0

6.0

8.0

2.0

Temperature

°C

30.0

33.5

37.0

3.5

Additive

PEG 6000 (g L− 1)

0.0

2.5

5.0

2.5