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Table 4 Natural levels and interval of variation in the independent variables in the design of cellulose production

From: Optimized culture conditions for bacterial cellulose production by Acetobacter senegalensis MA1

Factors Codes Levels Interval of variation
-1 0 + 1
Carbon Glycerol (mL L− 1) 45.0 50.0 55.0 5.0
Nitrogen Yeast extract (g L− 1) 5.0 7.5 10.0 2.5
pH   4.0 6.0 8.0 2.0
Temperature °C 30.0 33.5 37.0 3.5
Additive PEG 6000 (g L− 1) 0.0 2.5 5.0 2.5