Loop-mediated isothermal amplification (LAMP) reaction as viable PCR substitute for diagnostic applications: a comparative analysis study of LAMP, conventional PCR, nested PCR (nPCR) and real-time PCR (qPCR) based on Entamoeba histolytica DNA derived from faecal sample

Table 2 Oligonucleotide sequences used in this study

Primers	Sequences (5′-3′)	Length (mer)	Product size
Loop-Mediated Isothermal Amplification
Eh-FIP-SER	GCTTCGTTCTTTAAAAATACACCGTCATTCTTGATTTGGATCAAGAAGT	49	–
Eh-BIP-SER	AGTAGCTCAGCAAAACCAGAATCACTTGCTTTTTCATCTTCATCA	45
Eh-F3-SER	TGCATTCACTAGTGCAACT	19
Eh-B3-SER	GCTTGATTCTGAGTTATCACTTG	23
Eh-LB-SER	AAGTTCAAATGAAGATAATGAA	22
Conventional PCR & Real-Time PCR
Eh-F2	CATTCTTGATTTGGATCAAGAAGT	24	175 bp
Eh-B2	ACTTGCTTTTTCATCTTCATCA	22	175 bp
Nested PCR
Eh-F3-SER	TGCATTCACTAGTGCAACT	19	223 bp
Eh-B3-SER	GCTTGATTCTGAGTTATCACTTG	23	223 bp
Eh-F2	CATTCTTGATTTGGATCAAGAAGT	24	175 bp
Eh-B2	ACTTGCTTTTTCATCTTCATCA	22	175 bp

ISSN: 1472-6750