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Fig. 8 | BMC Biotechnology

Fig. 8

From: Loop-mediated isothermal amplification (LAMP) reaction as viable PCR substitute for diagnostic applications: a comparative analysis study of LAMP, conventional PCR, nested PCR (nPCR) and real-time PCR (qPCR) based on Entamoeba histolytica DNA derived from faecal sample

Fig. 8

Spiked stool analytical sensitivity on LAMP using 10-fold dilutions of E. histolytica trophozoites and analysed using (a) agarose gel electrophoresis, (b) calcein-manganese dye and (c) LFD. L, 100 bp DNA ladder; N, negative control; 1–10, 10-fold dilution of trophozoites: lane 1, 106; lane 2, 105; lane 3, 104; lane 4, 103; lane 5, 102; lane 6, 10; lane 7, 1; lane 8, 0.1; lane 9, 10− 2; lane 10, 10− 3; lane 11, 10− 4. All the 3 post-LAMP analyses recorded 1 trophozoite as LoD

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BMC Biotechnology

ISSN: 1472-6750

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