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Fig. 5 | BMC Biotechnology

Fig. 5

From: Loop-mediated isothermal amplification (LAMP) reaction as viable PCR substitute for diagnostic applications: a comparative analysis study of LAMP, conventional PCR, nested PCR (nPCR) and real-time PCR (qPCR) based on Entamoeba histolytica DNA derived from faecal sample

Fig. 5

Optimisation of LAMP (a) inner primers concentration and (b) outer primers concentration. L, 100 bp DNA ladder; B, blank (no DNA template control); N, negative control; 1–16, primer concentrations in μM: lane 1, 0.33; lane 2, 0.67; lane 3, 1.0; lane 4,1.33; lane 5, 1.67; lane 6, 2.0; lane 7, 2.33; lane 8, 2.67; lane 9, 0.083; lane 10, 0.10; lane 11, 0.117; lane 12, 0.133; lane 13, 0.167; lane 14, 0.25; lane 15, 0.33; lane 16, 0.67. The selected optimum inner primers concentration was 2.0 μM while 0.167 μM was selected as optimum outer primers concentration

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BMC Biotechnology

ISSN: 1472-6750

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