Fig. 4
From: Evaluation of Hydra HALT-1 as a toxin moiety for recombinant immunotoxin
ELISA assay of CD64 binding to recombinant immunotoxins. a HALT-1-scFv; b scFv-HALT-1; c HALT-1. Various concentrations of HALT-1-scFv, scFv-HALT-1 and HALT-1 (0, 1, 2, 4, 8 and 10 μg/mL) were used in the assay with or without CD64. Each assay was performed in triplicate and error bars represent standard deviations from the mean of triplicate. Recombinant HALT-1 alone was used as a control to indicate that non-specific binding did not occur between HALT-1 and CD64. Fluorescence intensity was obtained by subtracting the fluorescence value with 0 μg/mL of either HALT-1-scFv, scFv-HALT-1 or HALT-1