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Table 2 Plasmids used in this study (from Addgene, USA)

From: Efficient CRISPR/Cas9 genome editing in a salmonid fish cell line using a lentivirus delivery system

Plasmid construct

Plasmid name (Addgene)

Plasmid number (Addgene)

pLenti CMV:EGFP_PGK:Puro

pLenti CMV GFP Puro (658–5)

17,448, [43]

pLenti hU6:gRNA_PKG:Puro

pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W

67,974, [21]

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BMC Biotechnology

ISSN: 1472-6750

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