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Table 3 Phenotype and the detected mutations in the PDS-mutated potato plants

From: Generation of transgene-free PDS mutants in potato by Agrobacterium-mediated transformation

Tf. organSelectionStrainMutantPheno
type
Genotype*
Allele 1Allele 2
(−AC)
Allele 3
(+ 16 bp)
Allele 4
(variant)
TuberKmgv2260PDS.12 ggreenΔ165 (2) Δ5 (2) Δ165 (1) 
PDS.5 mmosaic
(tabby)
Δ1 (1)
wt (2)
 wt (1)Δ165 (7)
PDS.3wwhite   Δ146 (1)
Δ166 (2)
PDS.11wwhiteΔ104 (2)   
LBA4404PDS.28 ggreenΔ35 + T/A (5)
wt (1)
   
PDS.34 ggreen wt (1) Δ165 (4)
Tuber3d-Kmgv2260PDS.73 ggreenwt (3)wt (2)Δ1 (1) 
3d-Km
/5-FC
PDS.65 ggreenΔ1 (1)
wt (3)
wt (1)  
3d-KmLBA4404PDS.48 ggreenΔ2 + T/A (1)
wt (1)
wt (2)  
PDS.9 mmosaic
(tabby)
Δ3 (1)Δ1 (3)wt (3) 
3d-Km /5-FCPDS.10 mmosaic
(patchy)
wt (3)  Δ117 (1)
Leaf3d-Kmgv2260PDS.10 ggreenΔ1 + Δ3 (1)
wt (2)
wt (1)  
3d-KmLBA4404PDS.1 ggreenΔ2 (1)
wt (6)
   
PDS.33 ggreenΔ2 + G/A (1)
wt (8)
   
3d-Km /5-FCPDS.5 ggreenwt (3)Δ2 + T/C (1)
wt (1)
  
  1. *Δ numbers indicate the size of the deletions in bp. Numbers in parenthesis specify the number of clones with identical sequence. /, base pair changes; wt, wild-type