Skip to main content

Table 1 Efficiency of PDS mutagenesis with Km selection

From: Generation of transgene-free PDS mutants in potato by Agrobacterium-mediated transformation

Transformed organStrainVectorNo. of regenerated plantsNo. of tested plantsaNo. of mutantsEfficiencyb (%)
Tubergv2260CP02536161169
PROGED248788
LBA4404CP02556211886
PROGED2817847
Leafgv2260PROGED2414964
LBA4404169778
  1. a Plants for testing the presence of mutation in the PDS gene were selected randomly
  2. b Efficiency (%) of PDS mutagenesis was calculated as number of mutants detected by the restriction enzyme site loss method in PCR amplification of genomic DNA after digestion with MlyI / number of tested plants × 100