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Table 1 HOIP-Chaperone combinations created with the GoldenBac cloning system

From: GoldenBac: a simple, highly efficient, and widely applicable system for construction of multi-gene expression vectors for use with the baculovirus expression vector system

Construct nameGene 1Gene 2Gene 3Gene 4Gene 5
TS-HOIPTS-HOIP
HC1TS-HOIPF-HSPA8 
HC2TS-HOIPF-HSPA8F-DNAJA1
HC3TS-HOIPF-DNAJA1
HC4TS-HOIPF-HSPA8F-DNAJA1His-HSPH2
HC5TS-HOIPF-HSPA1AF-DNAJA1
HC6TS-HOIPF-HSPA8F-DNAJB1
HC7TS-HOIPF-DNAJA1His-DNAJB1
HC8TS-HOIPF-HSPA8F-DNAJA1His-DNAJB1
HC9TS-HOIPF-HSPA8F-DNAJA1His-HIPHis-Hsp90AA1
HC10TS-HOIPF-sfHSPA8F-sfDNAJA1
  1. TS TwinStrep affinity tag, F FLAG epitope tag, His His6 affinity tag, sf Spodoptera frugiperda. All genes were expressed under the control of the polH promoter, except for DNAJA1, which was under the control of the p10 promoter