Skip to main content

Table 1 HOIP-Chaperone combinations created with the GoldenBac cloning system

From: GoldenBac: a simple, highly efficient, and widely applicable system for construction of multi-gene expression vectors for use with the baculovirus expression vector system

Construct name

Gene 1

Gene 2

Gene 3

Gene 4

Gene 5

TS-HOIP

TS-HOIP

HC1

TS-HOIP

F-HSPA8

 

HC2

TS-HOIP

F-HSPA8

F-DNAJA1

HC3

TS-HOIP

F-DNAJA1

HC4

TS-HOIP

F-HSPA8

F-DNAJA1

His-HSPH2

HC5

TS-HOIP

F-HSPA1A

F-DNAJA1

HC6

TS-HOIP

F-HSPA8

F-DNAJB1

HC7

TS-HOIP

F-DNAJA1

His-DNAJB1

HC8

TS-HOIP

F-HSPA8

F-DNAJA1

His-DNAJB1

HC9

TS-HOIP

F-HSPA8

F-DNAJA1

His-HIP

His-Hsp90AA1

HC10

TS-HOIP

F-sfHSPA8

F-sfDNAJA1

  1. TS TwinStrep affinity tag, F FLAG epitope tag, His His6 affinity tag, sf Spodoptera frugiperda. All genes were expressed under the control of the polH promoter, except for DNAJA1, which was under the control of the p10 promoter