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Fig. 6 | BMC Biotechnology

Fig. 6

From: GoldenBac: a simple, highly efficient, and widely applicable system for construction of multi-gene expression vectors for use with the baculovirus expression vector system

Fig. 6

GoldenBac facilitates screening of chaperone co-expression constructs. Ten different baculoviruses expressing the TwinStrep-tagged HOIP (TS-HOIP) protein together with different combinations of chaperones were generated and HOIP expression levels were analyzed. Composition of the constructs HC1–10 are described in Table 1. a. Total cell lysates were analyzed by Western blotting to detect TS-HOIP and FLAG-tagged HSPA8, HSPA1A, sfHSPA8, or DNAJA1. The baculovirus protein gp64 was used as a loading control. b. Soluble fractions were also analyzed by Western blotting for TS-HOIP. c. SDS-PAGE analysis of the purification of TS-HOIP alone (left) or of construct HC2, which contains TS-HOIP co-expressed with HSPA8 and DNAJA1 (right). HSPA8 is visible as a contaminant in the elution

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BMC Biotechnology

ISSN: 1472-6750

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