Purification of the APC/C using a GoldenBac generated virus. The APC/C was purified using a three-step procedure consisting of Strep-Tactin affinity chromatography followed by ion exchange and size exclusion. The final protein complex isolated by size exclusion was analyzed by SDS-PAGE on a 4–12% Bis-Tris NuPAGE minigel and stained with Coomassie blue. All components of the APC/C can be identified, as indicated to the right of the gel. Lane 1, molecular weight marker; lane 2, 2 μg of the final purified APC/C complex.