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Fig. 4 | BMC Biotechnology

Fig. 4

From: GoldenBac: a simple, highly efficient, and widely applicable system for construction of multi-gene expression vectors for use with the baculovirus expression vector system

Fig. 4

Purification of the APC/C using a GoldenBac generated virus. The APC/C was purified using a three-step procedure consisting of Strep-Tactin affinity chromatography followed by ion exchange and size exclusion. The final protein complex isolated by size exclusion was analyzed by SDS-PAGE on a 4–12% Bis-Tris NuPAGE minigel and stained with Coomassie blue. All components of the APC/C can be identified, as indicated to the right of the gel. Lane 1, molecular weight marker; lane 2, 2 μg of the final purified APC/C complex.

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BMC Biotechnology

ISSN: 1472-6750

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