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Table 1 Antimicrobial activity of the chimeric peptide evaluated by disc diffusion method

From: Generation of an engineered food-grade Lactococcus lactis strain for production of an antimicrobial peptide: in vitro and in silico evaluation

Bacterial speciesVolume of supernatant (μL)Positive controlNegative control
102040Gentamicin
S. aureus11.1 ± 0.14 c15.8 ± 0.14 b21.1 ± 0.14a20.1 ± 0.14 a_
E. coli9.1 ± 0.17 c13.8 ± 0.17 b16.2 ± 0.17a15.8 ± 0.17a_
S. typhimurium11.2 ± 0.33c13.9 ± 0.33b18.4 ± 0.33a19.0 ± 0.33a_
L. monocytogenes11.1 ± 0.50 c16.6 ± 0.5 b21.3 ± 0.5 a21.3 ± 0.5 a_
E. faecalis20.8 ± 0.32 d24.5 ± 0.32 c28.6 ± 0.32 b30.1 ± 0.32 a_
P. aeroginosa21.0 ± 0.22d24.2 ± 0.22c27.6 ± 0.22 b31.1 ± 0.22 a_
  1. The data are the inhibition zone around the discs (mm) loaded by different volume of supernatants containing recombinant peptide and antibiotic. The data are presented as average values of three replicates with their standard error. Negative control: M17 cultured with non-recombinant L. lactis. Means with different letter(s) are significantly different based on DUNCAN multiple test (α = 0.05). – representing no growth inhibition