Fig. 2From: An efficient gene disruption method for the woody plant pathogen Botryosphaeria dothideaVerification of Bdo_05381-disrupted B. dothidea HTLW03 transformants by PCR. Transformants were analyzed with four PCR amplifications. The lanes marked with “+” correspond to the correct GD transformants with Bdo_05381 upstream and downstream fragments that were the correct size, an amplicon that was 400 bp longer than that of Bdo_05381, and no open reading frame fragmentBack to article page