Fig. 5
Inhibition of C. albicans growth by extracts or culture medium of N. benthamiana scFvFc 2G8 HR. Candida albicans cells were cultured at 37 °C in the presence of the indicated HR concentrated extracts. aCandida albicans cells (103/mL) were cultured at 37 °C in the presence of concentrated extracts of N. benthamiana HR expressing (HR Cl 7) or not expressing (HR C-, negative control) the 2G8 antibody. b The same number of fungal cells were cultured in presence of a concentrated culture medium of N. benthamiana HR expressing the 2G8 antibody (HR Cl 7, final antibody concentration 150 μg/mL) or with an identically concentrated culture medium of N. benthamiana HR C- (negative control) or with a scFvFc 2G8 purified from N. benthamiana HR (scFvFc 2G8 HR pur, final antibody concentration 200 μg/mL). When indicated (+ scFvFc 2G8), the scFvFc 2G8 purified from N. benthamiana leaves (final concentration 200 μg/mL) was added to HR concentrated culture media. All conditions were assayed in triplicate. After 24 h of incubation, fungal growth was estimated by enumerating fungal cells with a standard CFU counts. Values in the graphs are mean numbers of fungal cells found in the different cultures at the end of the incubation period. Percent growth inhibition values were calculated by comparison to negative controls (cultures growth in the presence of HR C- preparations) and are reported at the end of the relevant bar. Statistically significant differences (P < 0.05, two-tailed Student’s t test) with respect to negative controls are marked by asterisks