Fig. 4

Screening of markers and mutations in the transgenic series targeting Glyma.18 g041100. a) The sequences at the gRNA target site for the transgenic plants and Bert-MN-01 control. Sequences from the T0 plant are labeled as WPT553–6 and the lower sequences (labeled WPT553–6-8 and WPT553–6-11) are from the T1 progeny. Dashes represent a deletion within a sequence. Percentages indicate the proportion of reads for each of the mutations in the respective plants. b) PCR assay screening for presence/absence of the Cas9 endonuclease, BAR plant-selectable marker, and actin control. Lane are labeled as the transformation vector control (top row and far left for each assay), the T0 plant (WTP553–6), the subsequent generation (WPT553–6-11), and the following generation (WPT553–6-11-x plants)