Fig. 2

Whole-genome sequencing results of the transgenic series targeting GmRin4b. a) The sequences of transgenic plants and the Bert-MN-01 control at gRNA target site are shown. Sequences labeled ‘WPT536–2’ are from the T0/M0 plant while the bottom two sequences are from the M2 progeny. Dashes represent a deletion within a sequence, while red text indicates an insertion. Percentages represent the proportion of reads in a given sample showing each respective sequence. b) The diagram depicts WGS detection of the transgene insertion event and the variation induced at the insertion site. The blue to red gradient represents a color map of a transgene cassette to associate which segments of the transgene are integrated into the genome. The colors flanking the unresolved transgene structure are integration sites identified through sequencing associate to areas of the transgene colormap with orange representing an addition