Fig. 3
From: Development and applications of a monoclonal antibody against caprine interferon-gamma
a Generation and specificity determination of mAb 2C by ELISA. For the ELISA analysis, purified rIFN-γ protein (1 μg), fusion tag protein of pET-32a (1 μg), Con A-stimulated or non-stimulated goat or sheep PBMCs were served as antigens. In addition, the culture supernatant of 2C hybridoma cells was served as the primary antibody (n = 6, ***P < 0.001). b Western blot analysis of reactivity of mAb 2C. M, protein marker; lane 1, rIFN-γ protein; lane 2, fusion tags protein of pET-32a