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Fig. 4 | BMC Biotechnology

Fig. 4

From: A self-aggregating peptide: implications for the development of thermostable vaccine candidates

Fig. 4

The PH(1–110) GFP particles induce immune response against GFP without the use of adjuvant. a Immunization scheme of mice in which two immunizations are included on day 0 and 15, the blood sample was taken for 24 weeks at 15-day intervals. b First evaluation of the immune response induced by PH(1–110) GFP particles of different sizes obtained in the sucrose gradients. In the immunized mice the production of IgG against GFP was measured by ELISA. All groups were compared with the particles mix group at week 10. c The antibody response against GFP was compared between particles mix of PH(1–110) GFP with and without Alum and free GFP with and without Alum. d Serial 2-fold dilution of the sera of the mice to evaluate the antibody titers on week 4. The gray line indicates the cut-off. Error bars indicate the means ± SD (n = 5). *** p < 0.001, ns = not significant. (For panel B, two-way ANOVA with Dunnett post-tests and for panel C was used two-way ANOVA with Tukey post-tests)

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